Candid conversations with the big hitters of flow cytometry presented by Dr Peter O'Toole. Brought to you by Beckman Coulter Life Sciences and Bitesize Bio. Flow Stars is a podcast from Beckman Coulter Life Sciences and Bitesize Bio that interviews some big hitters in the flow cytometry world. Your host is Dr Peter O'Toole (University of York), an engaging and energic personality who knows how to throw a few curveballs to keep his guest on their toes. This series takes us through the highs and lows of life in flow cytometry, looking back at guests' careers and forward to the future of this technology. Whether you already have your feet firmly in the field or are just cheering from the side lines, each episode entertains, informs, and inspires in equal measure. https://bitesizebio.com/flowstars
In this episode, Peter O'Toole is joined by Virginia Litwin, the President-Elect of the International Society For Advancement of Cytometry (ISAC).
They discuss Virginia's journey from academia to industry and her pivotal role in the creation and development of CYTO Women, a committee that's having a genuine positive impact on the opportunities available for women in cytometry.
She also shares her experiences in translational...
In this episode of Flow Stars, we're joined by Anis Larbi, Medical & Scientific Affairs, Beckman Coulter Life Sciences.
Anis chats about his experience working at the prestigious A*STAR in Singapore, where he led a dual career as both a principal investigator and the head of a core flow cytometry facility.
He also shares his advocacy of networking, explains this inspired him to make the leap from academia to industry an...
In this episode, Peter O'Toole welcomes Sofie Van Gassen, a Postdoctoral Researcher from VIB-UGent. Sofie explains how a computer scientist can end up working in flow cytometry and sets out her vision for the future of data science in flow. She also sets out her twin passions for developing algorithms and pipelines for data analysis—and gardening.
Watch or listen to all episodes of Flow Stars: flowstars.bitesizebio.com
...Today, Peter O'Toole chats with Dave Novo-Lake, founder of De Novo Research, a pioneering figure in flow cytometry data analysis, and the creator of FCS Express. Dave shares his journey from developing groundbreaking software in his spare time at a Boston lab to establishing a successful business that transformed clinical flow cytometry.
He also talks about keeping his passion for science alive with special projects, his ex...
Today, our host Peter O'Toole sits down with the distinguished Mario Roederer, ImmunoTechnology Section Chief and Acting Head of the Translational Research Program at the National Institutes of Health.
Mario shares some fascinating insights from his illustrious career, from demonstrating flow cytometry to Presidents Barack Obama and George Bush to developing the widely used FlowJo™ software.
He also shares his inspirations,...
Let's shift our focus from just the people behind the science to the technologies they're using.
In this special episode, we dive deep into spectral flow cytometry with a panel of leading experts, featuring:
• Tamar Tak, Coordinator Flow Cytometry Facility, Leiden University Medical Center
• Domenico Lo Tartaro, University of Modena and Reggio Emilia
• Andrew Patentreger, Senior Team Lead, Broad Institute of MIT and Ha...
In this special episode of Flow Stars recorded live at CYTO 2024, our panel of esteemed guests, representing a cross-section of cytometry users, discuss the biggest matters in flow cytometry.
They explore the capabilities of current cytometers and what future developments should focus on. They also consider the importance of making cytometry equipment more affordable and the level of technical support provided by manufactu...
#S4:E6 — Karen Hogg is a Technical Specialist at the University of York, and Margaret Mbuchi is a Principal Research Scientist at the Kenya Medical Research Institute.
In this episode of Flow Stars, Karen and Margaret join Peter O’Toole to celebrate the achievements of flow cytometry initiatives across Africa and discuss some of the logistical challenges involved. Karen shares some of the logistical and technical hurdles i...
#S4:E5 — Alexis Perez Gonzalez is the Melbourne Cytometry Platform Manager at the University of Melbourne. In this episode of Flow Stars, Alexis joins Peter O’Toole to discuss how academic progression works in Cuba and how much fun he had working with Andy Riddell. They also chat about his magnificent collection of spices and cookbooks.
Watch or listen to all episodes of Flow Stars: flowstars.bitesizebio.com
#S4:E4 — Ryan Brinkman is Professor Emeritus at the British Columbia Cancer Agency and Chair of the International Society for Advancement of Cytometry's (ISAC) Data Standards Task Force.
In this episode of Flow Stars, Ryan joins Peter O’Toole to discuss generating data without a wet lab. They also discuss bioinformatics' clinical and translational benefits and consider what motivates academics.
#S4:E3 — Jessica Back is Assistant Professor - Research at the Karmanos Cancer Institute at Wayne State University. In this episode of Flow Stars, Jessica joins Peter O’Toole to discuss her difficulties getting through grad school. They also chat about places you can talk science without feeling like the odd one out and the time she had to process a spleen in front of a news crew.
#S4:E2 — Peter Lopez is Associate Professor in the Department of Pathology at New York University and former Director of the Cytometry and Cell Sorting Laboratory. In this episode of Flow Stars, Peter joins Peter O’Toole to discuss the benefits of semi-retirement.
They also chat about his side hustle making pizza and maple syrup. Watch or listen to all episodes of Flow Stars: flowstars.bitesizebio.com
#S4:E1 — In this special episode of Flow Stars recorded live at CYTO 2023, host Peter O'Toole welcomes a panel of distinguished experts, including Jessica Houston (New Mexico State University), Pratip Chattopadhyay (NYU Langone Health (PIL)), and Paul Wallace (Roswell Park Cancer Comprehensive Center).
Join them for an enlightening conversation as they dive into the future of flow cytometry and provide their unique perspect...
#S3:E6 — Sara De Biasi and Andrea Cossarizza from the University of Modena and Reggio Emilia (UNIMORE) chat with Peter O'Toole about how they were able to quickly respond to news of a novel coronavirus as early as January 2020, and how their work was crucial to understanding the effect of COVID-19 on the immune system.
They also discuss the pros and cons of moving abroad or staying where you are to pursue a career, as well ...
#S3:E5 — Jessica Houston chats to Peter O'Toole about developing her own niche as a new PI, while balancing this with a busy family home life. She discusses her new appointment as president-elect for the International Society for Advancement of Cytometry (ISAC), and the important role societies can play in science. She recalls her early career developing her research, and how she knew industry wasn't for her!
Jessica also c...
#S3:E4 — Bill Telford is Head of the NCI Research Flow Facility at the NCI Center for Cancer Research.
In this episode of Flow Stars, Bill joins Peter O'Toole to discuss his early career inspirations, his passion for supporting international instrument donation and training initiatives, some of the challenges he's faced running a flow core facility, and being a bit of a science geek—he builds his own flow cytometers using 3...
#S3:E3 — Andrea Holme is Head of the Iain Fraser Cytometry Centre at the University of Aberdeen.
In this episode of Flow Stars, Andrea joins Peter O'Toole to discuss how she was able to use her passion for traveling to her advantage during her varied scientific career. They also chat about the key differences between industry and academia, why she thinks it's important to be involved in different scientific societies, and a...
#S3:E2 — Grace Chojnowski is Flow Cytometry and Imaging Facility Manager at the Queensland Institute of Medical Research.
In this episode of Flow Stars, Grace joins Peter O'Toole to discuss why understanding the theory behind your experiment is key in flow cytometry, why she'd like a flow lab in the Alps, and her early career as an Australian living in London. We also hear about a funny interview story and talk candidly abo...
#S3:E1 — John Tigges joins Peter O'Toole in the latest episode of Flow Stars to discuss his passion for training the next generation of scientists in flow cytometry, his hobbies of DJing and MCing, and managing the business side of a core facility. We also hear why he thinks people who work in core facilities are often like the bartenders of the science world—and how he has often heard more than he should have!
#S2:E6 — Paul K. Wallace of the Roswell Park Comprehensive Cancer Center was one of the first clinical cytometrists. He has had a huge influence on the field of flow cytometry, particularly in developing assays to track immune function. In this engaging episode of Flow Stars, Paul chats to Peter O’Toole about his early ambitions to become a vet, the importance of education and training in flow cytometry, and how his role h...
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The Burden is a documentary series that takes listeners into the hidden places where justice is done (and undone). It dives deep into the lives of heroes and villains. And it focuses a spotlight on those who triumph even when the odds are against them. Season 5 - The Burden: Death & Deceit in Alliance On April Fools Day 1999, 26-year-old Yvonne Layne was found murdered in her Alliance, Ohio home. David Thorne, her ex-boyfriend and father of one of her children, was instantly a suspect. Another young man admitted to the murder, and David breathed a sigh of relief, until the confessed murderer fingered David; “He paid me to do it.” David was sentenced to life without parole. Two decades later, Pulitzer winner and podcast host, Maggie Freleng (Bone Valley Season 3: Graves County, Wrongful Conviction, Suave) launched a “live” investigation into David's conviction alongside Jason Baldwin (himself wrongfully convicted as a member of the West Memphis Three). Maggie had come to believe that the entire investigation of David was botched by the tiny local police department, or worse, covered up the real killer. Was Maggie correct? Was David’s claim of innocence credible? In Death and Deceit in Alliance, Maggie recounts the case that launched her career, and ultimately, “broke” her.” The results will shock the listener and reduce Maggie to tears and self-doubt. This is not your typical wrongful conviction story. In fact, it turns the genre on its head. It asks the question: What if our champions are foolish? Season 4 - The Burden: Get the Money and Run “Trying to murder my father, this was the thing that put me on the path.” That’s Joe Loya and that path was bank robbery. Bank, bank, bank, bank, bank. In season 4 of The Burden: Get the Money and Run, we hear from Joe who was once the most prolific bank robber in Southern California, and beyond. He used disguises, body doubles, proxies. He leaped over counters, grabbed the money and ran. Even as the FBI was closing in. It was a showdown between a daring bank robber, and a patient FBI agent. Joe was no ordinary bank robber. He was bright, articulate, charismatic, and driven by a dark rage that he summoned up at will. In seven episodes, Joe tells all: the what, the how… and the why. Including why he tried to murder his father. Season 3 - The Burden: Avenger Miriam Lewin is one of Argentina’s leading journalists today. At 19 years old, she was kidnapped off the streets of Buenos Aires for her political activism and thrown into a concentration camp. Thousands of her fellow inmates were executed, tossed alive from a cargo plane into the ocean. Miriam, along with a handful of others, will survive the camp. Then as a journalist, she will wage a decades long campaign to bring her tormentors to justice. Avenger is about one woman’s triumphant battle against unbelievable odds to survive torture, claim justice for the crimes done against her and others like her, and change the future of her country. Season 2 - The Burden: Empire on Blood Empire on Blood is set in the Bronx, NY, in the early 90s, when two young drug dealers ruled an intersection known as “The Corner on Blood.” The boss, Calvin Buari, lived large. He and a protege swore they would build an empire on blood. Then the relationship frayed and the protege accused Calvin of a double homicide which he claimed he didn’t do. But did he? Award-winning journalist Steve Fishman spent seven years to answer that question. This is the story of one man’s last chance to overturn his life sentence. He may prevail, but someone’s gotta pay. The Burden: Empire on Blood is the director’s cut of the true crime classic which reached #1 on the charts when it was first released half a dozen years ago. Season 1 - The Burden In the 1990s, Detective Louis N. Scarcella was legendary. In a city overrun by violent crime, he cracked the toughest cases and put away the worst criminals. “The Hulk” was his nickname. Then the story changed. Scarcella ran into a group of convicted murderers who all say they are innocent. They turned themselves into jailhouse-lawyers and in prison founded a lway firm. When they realized Scarcella helped put many of them away, they set their sights on taking him down. And with the help of a NY Times reporter they have a chance. For years, Scarcella insisted he did nothing wrong. But that’s all he’d say. Until we tracked Scarcella to a sauna in a Russian bathhouse, where he started to talk..and talk and talk. “The guilty have gone free,” he whispered. And then agreed to take us into the belly of the beast. Welcome to The Burden.
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