#86 — qPCR primer design is a bit of science, a bit of magic, and a little bit of luck. In this episode, we cover the science of qPCR primer design, a cornerstone in conducting successful qPCR or RT-qPCR assays for gene expression analysis. [1] Get top tips and learn why dedicating time to crafting high-quality primers can save your experiment from poor data and resource wastage. [2]
We guide you through utilizing popular tools like NCBI's Primer-BLAST for your qPCR primer designs, alongside introducing alternative online resources to aid in your experimentation journey. [3] Whether you're beginning your research journey or looking to hone your skills further, this episode promises to equip you with the knowledge to navigate the complex yet captivating world of qPCR primer design proficiently.
Resources:
1. A Step-by-Step Guide to Designing qPCR Primers. Available at: https://bitesizebio.com/10041/designing-qpcr-primers/
2. 10 Tips for Consistent qPCR. Available at: https://bitesizebio.com/1118/10-tips-for-consistent-real-time-pcr-rtpcr/
3. NCBI tool Primer-BLAST. Available at: https://www.ncbi.nlm.nih.gov/tools/primer-blast/
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