#88 — Getting the best out of your in situ hybridizations requires choosing the correct protocol, deciding if sections or whole mount is better, using the right equipment, making fresh buffers, careful planning for all steps, optimizing your probe concentration, and taking the time to get the development step right. In other words, there are a lot of ways in situ hybridizations can go wrong!
This episode walks you through your first in situ hybridization and how to totally nail it! [1] When you've finished listening, why not check out our article on fluorescence in situ hybridization (FISH)? [2]
Resources:
1. How to Totally Nail Your First in situ Hybridization. Available at: https://bitesizebio.com/44651/how-to-totally-nail-your-first-in-situ-hybridization/
2. Do You Know Where Your mRNAs Are? Available at: https://bitesizebio.com/13391/its-10-am-do-you-know-where-your-mrnas-are/
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